At the end of their life cycle, proteins may be transported through the vesicles to the lysosome where they are finally degraded ( 2, 3). As an example, most secreted proteins are produced in the endoplasmid reticulum, modified in the golgi and then transported in vesicles via the secretory pathway to their subcellular or extracellular destination. Once expressed as a fluorescent protein fusion, it is possible to track your protein of interest from its production site to its final destination. You can take advantage of the many Fluorescent Proteins (FP) available for microscopy, by cloning your protein of interest into a vector encoding a fluorescent tag. Visualization can be achieved using fluorescent microscopy - even in living cells and whole organisms. The first step in analyzing the localization of your protein of interest is to make it detectable. Getting ready to determine the subcellular localization of your protein of interest Analyzing differences in protein localization and transport between healthy and diseased states can also provide interesting insights into disease mechanisms and protein function. However, much knowledge about functionality can be gained by understanding the location and transport of the protein within a living cell. When studying the function of a protein or its role in a disease, researchers often isolate proteins of interest and examine them using biochemical methods thus removing the context of the cell. Take a sneak peak inside a cell with the images from the Allen Cell Explorer ( 1). Rounded numbers can be confusing, because it seems like we don't know the exact count.The same way the human body is made up of organs, cells comprise compartments and structures, called organelles. If your user doesn't care about the details, let him ignore the rest.
The ISO standard does not specify the ordering or spacing. I always use the ISO 4217 currency designation, after the number, with a space, like 1,000 USD, which is 3,672.94 AED right now. Everybody knows what $1,000 means, but other currencies don't have such one-letter abbreviation. Showing different currencies on the same chart is an error, whether it's a linechart or stacked area chart. Monetary values should be converted to a common currency, like USD or AED. Imagine debugging a drop in traffic, and having to shift the times for each country's local timezone in your head. However, this is still preferable to showing times in local times on dashboards, as that is too error-prone.
So the weekly seasonality gets shifted around, per country/timezone. For example, countries that are ahead in time, their Monday morning usage peak will show up in Sunday UTC time. But this can still lead to weird artefacts. What about timezones? It's best to standardize on one timezone, preferably UTC, and show all dates in UTC time, without specifiying the timezone.